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Fluoroimmunoassays Using the NRL Array Biosensor

  • Joel P. Golden
  • Kim E. Sapsford
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

Array-based biosensor technology offers the user the ability to detect and quantify multiple targets in multiple samples simultaneously (Analytical Sciences 23:5–10, 2007). The NRL Array Biosensor has been developed with the aim of creating a system for sensitive, rapid, on-site screening for multiple targets of interest. This system is fluorescence-based, using evanescent illumination of a waveguide, and has demonstrated the use of both sandwich and competitive immunoassays for the detection of both high and low molecular weight targets, respectively. The current portable, automated system has demonstrated detection of a wide variety of analytes ranging from simple chemical compounds to entire bacterial cells, with applications in food safety, disease diagnosis, homeland security and environmental monitoring.

Key words

Fluorescence Immunoassay NRL array biosensor Total internal reflection Fluorescence Automated 

Notes

Acknowledgments

The views, opinions, and/or findings described in this chapter are those of the authors and should not be construed as official Department of the Navy positions, policies, or decisions.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joel P. Golden
    • 1
  • Kim E. Sapsford
    • 1
  1. 1.Center for Bio/Molecular Science & EngineeringUS Naval Research LaboratoryWashingtonUSA

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