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Simple Luminescence Detector for Capillary Electrophoresis

  • Antonio Segura-Carretero
  • Jorge F. Fernández-Sánchez
  • Alberto Fernández-Gutiérrez
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

The performance of a homemade, simple, fluorescence-induced capillary electrophoresis (CE) detector is described here. It is based on LED as excitation source, a bifurcated optical fibre as a waveguide and a CCD as a photodetector. The connection of all the components is fairly easy, even for non-experts. This detector provides a low cost and rapid system for the determination of high-quantum-yield native fluorescence compounds and fluorescence derivatised compounds by CE with direct fluorescence deter mination. R-phycoerythrin and B-phycoerythrin were used as models for native fluorescence compounds and amine labelled with FITC were set as models for the fluorescence derivatised ones. Detection limits of 0.50 and 0.64 μg/mL for R-phycoerythrin and B-phycoerythrin and 1.6 × 10−7 M for FITC-labelled 1,6-diaminohexane were achieved. The homemade LED-IF detector is not expected to displace the LIF-IF one, but offers another possibility and a cheaper way to solve simple analytical problems for determining biomolecules.

Key words

Light-emitting diode-induced fluorescence CCD detector Capillary electrophoresis Protein analysis Native fluorescence detection Phycobiliproteins Fluorescence-derivatised compounds FTIC-labelled amines 

Notes

Acknowledgments

This work was supported by the projects CTQ2005-01914/ BQU of the Ministry of Education and Science and RMN-666 from the Andalusian Government.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Antonio Segura-Carretero
    • 1
  • Jorge F. Fernández-Sánchez
    • 1
  • Alberto Fernández-Gutiérrez
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of SciencesUniversity of GranadaGranadaSpain

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