Label-Free Serodiagnosis on a Grating Coupler

  • Thomas Nagel
  • Ehrentreich-Förster Eva
  • Frank F. Bier
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)


The unique feature of the label-free measurement techniques for screening specific binding molecules against a certain ligand is that knowledge about the analyte is not required. Due to the direct monitoring of the binding event, no further detection step, e.g., by a fluorescently labeled antibody, is necessary. This technique enables not only the analysis of binding properties, but also applications in serodiagnosis and in primary screening in drug discovery. Especially when complex biological solutions such as blood serum are used as sample fluids, the minimization of unspecific attachment is the crucial point of the assay. In this chapter, the basic handling of the grating coupler as an example of a label-free transducer is described, together with a simple protocol to minimize unspecific attachment when measuring undiluted blood serum.

Key words

Label-free detection Grating coupler Blood serum Serodiagnosis Passivation of glass surfaces Protein coupling 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Thomas Nagel
    • 1
    • 2
  • Ehrentreich-Förster Eva
    • 1
  • Frank F. Bier
    • 1
    • 2
  1. 1.Department of Molecular Bioanalytics & BioelectronicsFraunhofer Institute for Biomedical Engineering, Branch Potsdam-GolmPotsdamGermany
  2. 2.Institute of Biochemistry and BiologyUniversity of PotsdamPotsdamGermany

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