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Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL

  • Xiao Suo Wang
  • Roland Stocker
Part of the Methods In Molecular Biology book series (MIMB, volume 477)

Abstract

Atherosclerosis is associated with dysfunctional HDL, and oxidation of HDL is thought to give rise to HDL becoming dysfunctional. Lipoprotein oxidation represents a complex series of processes that can be assessed by various methods. In general, oxidation mediated by 1-electron or radical oxidants gives rise to lipid hydroperoxides (LOOHs) as the primary product. These LOOHs may then undergo further reactions giving rise to secondary lipid oxidation products and/or oxidation of lipoprotein-associated proteins. Thus, LOOHs specifically oxidize Met residues of apolipoprotein (apo) A-I and A-II (the major proteins of HDL) to MetO. Here we describe an HPLC-based method to detect oxidized HDL containing specifically oxidized forms of apoA-I and apoA-II. This method may be useful to assess the early stages of HDL oxidation in biological samples.

Key words

Oxidized apolipoproteins oxidized HDL lipid peroxidation LOOH MetO antioxidants ultracentrifugation HPLC 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Xiao Suo Wang
    • 1
  • Roland Stocker
    • 1
  1. 1.Centre for Vascular Research, Bosch Institute and Discipline of PathologyThe University of SydneyAustralia

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