Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage

  • Michael J. Depew
Part of the METHODS IN MOLECULAR BIOLOGY™ book series (MIMB, volume 461)

1. Introduction

The evaluation of the ontogeny of the skeletodontal system of vertebrates is a critical endeavor for many evolutionary and developmental biologists for numerous reasons, including (1) the bones, cartilage, and teeth of vertebrates are clearly essential to their life; (2) historically, the primary structural source of evidence for the evolution of vertebrate organisms resides in the past preservation of these tissues; and (3) bones, cartilage, and teeth normally develop with great fidelity to the possessor's developmental blueprint, and they therefore provide an excellent three-dimensional readout of both normal and experimentally challenged developmental patterning mechanisms. The sections that follow are constructed to facilitate such an endeavor by providing protocols to achieve differential staining of bone (via Alizarin red) and cartilage (via Alcian blue) and resources for an anatomical context in which to interpret the results.

2. Materials

2.1. Materials for the...


Methylene Blue Alcian Blue Methyl Benzoate Differential Staining Benzyl Benzoate 
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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Michael J. Depew
    • 1
  1. 1.Department of Craniofacial DevelopmentKing's College LondonLondonUK

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