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In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase

  • Vladimir V. DidenkoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 682)

Abstract

The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier stages of apoptosis compared to the terminal transferase technique. The in situ labeling applications of T7 DNA polymerase are presented and summarized from the DNA damage detection standpoint. The detailed protocols are provided together with the discussion of their advantages and limitations.

Key words

DNA breaks Apoptosis detection T7 DNA polymerase DNA damage In situ labeling 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Departments of Neurosurgery and Molecular & Cellular BiologyBaylor College of Medicine, and Michael E. DeBakey VA Medical CenterHoustonUSA

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