Abstract
The Southern blotting technique was developed by E. Southern in 1975 (1) to facilitate the identification of specific sequences within a complex mixture of DNA fragments. The technique itself has been modified over the years and is now an extremely sensitive and fast analytical technique used in almost every molecular biology laboratory.
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References
Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503.
Thomas, P.S. (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77, 5201.
Rigby, P.W.J., Dieckmann, M., Rhodes, C, and Berg, P. (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J. Mol Biol. 113, 237.
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© 1986 The Humana Press Inc.
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Hall, A. (1986). Southern Blotting. In: Slater, R.J. (eds) Experiments in Molecular Biology. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-405-0_5
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DOI: https://doi.org/10.1007/978-1-60327-405-0_5
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-082-4
Online ISBN: 978-1-60327-405-0
eBook Packages: Springer Book Archive