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A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining

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Peptide Microarrays

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 570))

Abstract

There has recently been increased interest in the potential for microarray technologies to study protein networks in a whole cell system within a single experiment. Protein-detecting microarrays are composed of numerous agents immobilized within a tiny area on solid surfaces to capture targeted proteins and to detect interactions in a high-throughput fashion. In this chapter, in order to extend the usability of peptide microarrays, we describe a novel dry peptide microarray format to obtain protein fingerprint (PFP) data sets and a statistical PFP data manipulation technique to quantitatively analyze targeted proteins.

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Usui, K., Tomizaki, Ky., Mihara, H. (2009). A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_13

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  • DOI: https://doi.org/10.1007/978-1-60327-394-7_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-393-0

  • Online ISBN: 978-1-60327-394-7

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