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Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators)

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Rat Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 597))

Abstract

The use of transgenesis is relatively rare in rats, and this is because of the relative difficulty in adding foreign genes by the conventional methods. Gene knock out and knock in by the conventional techniques of homologous recombination remain difficult in rats. This situation would be less crucial if the gene constructs were more reliable for the expression of foreign genes. The present chapter describes the state of the art in vector design for various genetic modifications in rats.

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Author information

Authors and Affiliations

  1. Département de Physiologie Animale, Institut National de la Recherche Agronomique, Nouzilly, France

    Louis-Marie Houdebine

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  1. Louis-Marie Houdebine
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Corresponding author

Correspondence to Louis-Marie Houdebine .

Editor information

Editors and Affiliations

  1. ITERT-INSERM U643, CHU de Nantes, bd. Jean Monnet 30, Nantes, 44093, France

    Ignacio Anegon

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Houdebine, LM. (2010). Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators). In: Anegon, I. (eds) Rat Genomics. Methods in Molecular Biology, vol 597. Humana Press. https://doi.org/10.1007/978-1-60327-389-3_4

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  • DOI: https://doi.org/10.1007/978-1-60327-389-3_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-388-6

  • Online ISBN: 978-1-60327-389-3

  • eBook Packages: Springer Protocols

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