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Molecular Endocrinology pp 335–362Cite as

Transgenic Mouse Technology: Principles and Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 590))

Abstract

Introduction of foreign DNA into the mouse germ line is considered a major technical advancement in the fields of developmental biology and genetics. This technology now referred to as transgenic mouse technology has revolutionized virtually all fields of biology and provided new genetic approaches to model many human diseases in a whole animal context. Several hundreds of transgenic lines with expression of foreign genes specifically targeted to desired organelles/cells/tissues have been characterized. Further, the ability to spatio-temporally inactivate or activate gene expression in vivo using the “Cre-lox” technology has recently emerged as a powerful approach to understand various developmental processes including those relevant to molecular endocrinology. In this chapter, we will discuss the principles of transgenic mouse technology, and describe detailed methodology standardized at our institute.

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Acknowledgments

T.R.K. acknowledges funding support from NIH (NCRR Center for Biomedical Research Excellence Program Grant RR024214, HD04394, HD056082 and K-INBRE P20 RR016475) and The Hall Family Foundation, Kansas City, MO. H.W. is the recipient of a postdoctoral fellowship from the Biomedical Research Training Program at the Kansas University Medical Center.

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Authors and Affiliations

  1. Departments of Molecular & Integrative Physiology, Pathology and Laboratory Medicine, and Institute of Maternal and Fetal Biolgoy, Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS, USA

    T. Rajendra Kumar

  2. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA

    Melissa Larson

  3. Department of Molecular and Integrative Physiology, Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS, USA

    Huizhen Wang

  4. Transgenic and Gene-Targeting Institutional Facility, Universityof Kansas Medical Center, Kansas City, KS, USA

    Jeff McDermott & Illya Bronshteyn

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  1. T. Rajendra Kumar
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  2. Melissa Larson
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  3. Huizhen Wang
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  4. Jeff McDermott
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  5. Illya Bronshteyn
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Editors and Affiliations

  1. College of Medicine, University of Kentucky, Rose Street 800, Lexington, 40536-0298, U.S.A.

    Ok-Kyong Park-Sarge

  2. Dept. Obstetrics & Gynecology, University of Kentucky, Rose Street 800, Lexington, 40536-0293, U.S.A.

    Thomas E. Curry

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Kumar, T.R., Larson, M., Wang, H., McDermott, J., Bronshteyn, I. (2009). Transgenic Mouse Technology: Principles and Methods. In: Park-Sarge, OK., Curry, T. (eds) Molecular Endocrinology. Methods in Molecular Biology, vol 590. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-378-7_22

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  • DOI: https://doi.org/10.1007/978-1-60327-378-7_22

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