Skip to main content
SpringerLink
Log in

Biomedical Uses of Flow Cytometry

  • Protocol
Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Shapiro H (2003) History. In: Practical flow cytometry, 4th edn, John Wiley, Hoboken, New Jersey

    Book  Google Scholar 

  2. Herzenberg LA, Parks D, Sahaf B, Perez O, Roederer M, Herzenberg LA (2002) The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford. Clin Chem 48:1819–1827

    PubMed  CAS  Google Scholar 

  3. Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) (2001) WHO classification, pathology and genetics tumors of haematopoietic and lymphoid tissues. IARC, Lyon, France

    Google Scholar 

  4. Tbakhi A, Edinger M, Myles J, Pohlman B, Tubbs RR (1996) Flow cytomet-ric immunophenotyping of non-Hodgkin's lymphoma and related disorders. Cytometry 25:113–124

    Article  PubMed  CAS  Google Scholar 

  5. Weir EG, Borowitz MJ (2001) Flow cytometry in the diagnosis of acute leukemia. Semin Hematol 38:124–138

    Article  PubMed  CAS  Google Scholar 

  6. Fukushima PI, Nguyen PK, O'Grady P, Stetler-Stevenson M (1996) Flow cytomet-ric analysis of kappa and lambda light chain expression. Commun Clin Cytometry, 26:243–252

    Article  CAS  Google Scholar 

  7. Li S, Eshleman JR, Borowitz MJ (2002) Lack of surface immunoglobulin light chain expression by flow cytometric immunophenotyping can help diagnose peripheral B cell lymphoma. Am J Clin Pathol 118:229–234

    Article  PubMed  CAS  Google Scholar 

  8. Kuchnio M, Sausville EA, Jaffe ES, Greiner T, Foss FM, McClanahan J, Fukushima P, Stetler-Stevenson MA (1994) Flow cytometric detection of neoplas-tic T cells in patients with mycosis fungoides based upon levels of T-cell receptor expression. Am J Clin Pathol 102:856–860

    PubMed  CAS  Google Scholar 

  9. Edelman J, Meyerson HJ (2000) Diminished CD3 expression is useful for detecting and enumerating Sezary cells. Am J Clin Pathol 114:467–477

    PubMed  CAS  Google Scholar 

  10. Gorczyca W, Weisberger J, Liu Z, Tsang P, Hossein M, Wu CD, Dong Wong JYL, Tugulea S, Dee S, Melamed MR, Darzynkiewicz Z (2002) An approach to diagnosis of T-cell lymphoproliferative disorders by flow cytometry. Cytometry (Clin Cytometry) 50:177–190

    Article  Google Scholar 

  11. van den Beemd RB, Boor PPC, van Lochem EG, Hop WCJ, Langerak AW, Wolvers-Tettero ILM, Hooijkaas H, van Dongen JJM (2000) Flow cytometric analysis of the V beta repertoire in healthy controls. Cytometry 40(4):336–345

    Article  PubMed  Google Scholar 

  12. Lima M, Almeida J, Santos AH, dos Anjos Teixeira M, Alguero MC, Queiros ML, Balanzategui A, Justica B, Gonzalez M, San Miguel JF, Orfao A (2001) Immunophenotypic analysis of the TCR-V repertoire in 98 persistent expansions of CD3+/TCR large granular lymphocytes. Am J Pathol 159:1861–1868

    Article  PubMed  CAS  Google Scholar 

  13. Weir EG, Borowitz MJ. (2001) Flow cytometry in the diagnosis of acute leukemia. Semin Hematol 38:124–138

    Article  PubMed  CAS  Google Scholar 

  14. Borowitz MJ, Guenther KL, Shults KE, Stelzer GT (1993) Immunophenotyping of acute leukemia by flow cytometric analysis: use of CD45 and right angle light scatter to gate on leukemic blasts in three color analysis. Am J Clin Pathol 100:534–540

    PubMed  CAS  Google Scholar 

  15. Ranier R, Hodges I, Stelzer G (1995) CD45 gating correates with bone marrow differential. Cytometry 22:139–145

    Article  Google Scholar 

  16. Stetler-Stevenson M, Schrager JA (2006) Flow cytometry In: Keren DF, McCoy JP, CareyJL (eds), Clinical Diagnosis, 4th edn., ASCP, Chicago 129–167

    Google Scholar 

  17. Garratty G, Arndt PA (1999) Applications of Flow Cytofluorometry to Red Blood Cell Immunology. Cytometry 38:259–267

    Article  PubMed  CAS  Google Scholar 

  18. Davis BH, Olsen S, Bigelow NC, Chen JC (1998) Detection of fetal red cells in fetomaternal hemorrhage using a fetal hemoglobin monoclonal antibody by flow cytometry. Transfusion 38:749–756

    Article  PubMed  CAS  Google Scholar 

  19. Ault KA (2001) The clinical utility of flow cytometry in the study of platelets. Semin Hematol 38:160–168

    Article  PubMed  CAS  Google Scholar 

  20. Davis B, Bigelow N (1999) Indirect immunoplatelet counting by flow cytometry as a reference method for platelet count calibration. Lab Mematol 5:15–21

    Google Scholar 

  21. Tschoepe D, Schultheiss HP, Kolarov P, Schwippert B, Dannehl K, Nieuwenhuis HK, Kehrel B, Strauer B, Gries FA (1993) Platelet membrane activation markers are predictive for increased risk of acute ischemic events after PTCA. Circulation 88:37–42

    PubMed  CAS  Google Scholar 

  22. His ED (2000) Paroxysmal nocturnal hemaglobinuria testing by flow cytometry – Evaluation of the REDQUANT and CELLQUANT kits. AJCP 114:798–806

    Google Scholar 

  23. Hernandez-Campo PM, Martin-Ayuso M, Almeida J, Lopez A, Orfao A (2002) Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets. Cytometry 50:191–201

    Article  PubMed  Google Scholar 

  24. Horsbugh T, Martin S, Robson AJ (2000) The application of flow cytometry to histocompatibility testing. Transplant Immunol 8:3–15

    Article  Google Scholar 

  25. Kerman RH, Gebel H, Bray R, Garcia C, Sahai R, Gibson H, Radovancevic B, Knight R, Katz S, VanBuren C, Kahan B. (2002) HLA antibody and donor reactivity define patients at risk for rejection or graft loss. Tissue Antigens 59:60–660

    Article  Google Scholar 

  26. Bryan CF, McDonald SB, Baier KA, Luger AM, Aeder MI, Murillo D, Muruve NA, Nelson PW, Shield CF 3rd, Warady BA (2002) Flow cytometry beads rather than the antihuman globulin method should be used to detect HLA Class I IgG antibodies (PRA) in cadaveric renal regraft candidates. Clin Transpl 16:15–23

    Article  Google Scholar 

  27. Davis BH, Olsen SH, Ahmad E, Bigelow NC (2006) Neutrophil CD64 is an improved indicator of infection or sepsis in Emergency department patients. Arch Pathol 130:654–661

    CAS  Google Scholar 

  28. Mandy F, Nicholson J, Autran B, Janossy G (2002) T-cell subset counting and the fight against AIDS: reflections over a 20-year struggle. Cytometry (Clin Cytometry) 50:39–45

    Article  Google Scholar 

  29. Giorgi JV, Lyles RH, Matud JL, Yamashita TE, Mellors JW, Hultin LE, Jamieson BD, Margolick JB, Rinaldo Jr CR, Phair JP, Detels R. (2002) Predictive value of immunologic and virologic markers after long or short duration of HIV-1 infection. J Acq Imm Def Dis 29:346–355

    Google Scholar 

  30. O'Gorman MR, Scholl PR (2002) Role of flow cytometry in the diagnostic evaluation of primary immunodeficiency disease. Clin Appl Immunol Rev 2:321–335.

    Article  Google Scholar 

  31. Bleesing JJH. (2003) Autoimmune lymphoproliferative syndrome (ALPS). Curr Pharm Design 9:265–278

    Article  CAS  Google Scholar 

  32. Ebo DG, Sainte-Laudy J, Bridts CH, Mertens CH, Hagendorens MM, Schuerwegh AJ, De Clerk LS, Stevens WJ (2006) Flow-assisted allergy diagnosis: current and future perspectives. Allergy 61:1028–1039

    Article  PubMed  CAS  Google Scholar 

  33. Bienvenu J, Monneret G, Fabien N, Revillard JP. (2000) The clinical usefulness of the measurement of cytokines. Clin Chem Lab Med 38:267–285

    Article  PubMed  CAS  Google Scholar 

  34. Tricot G, Jagannath S, Vesole D, Nelson J, Tindle S, Miller L, Cheson B, Crowley J, Bariogi B (1995) Peripheral blood stem cell transplants for multiple myeloma: identification of favorable variables for rapid engraftment in 225 patients. Blood 85:588–596

    PubMed  CAS  Google Scholar 

  35. Weaver CH, Hazelton B, Birch R, Palmer P, Allen C, Schwartzberg L, West W (1995) An analysis of engraftement kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy. Blood 86:3961–3969

    PubMed  CAS  Google Scholar 

  36. Sutherland DR, Keating A, Nayar R, Anania S, Stewart AK (1994) Sensitive detection and enumeration of CD34+ cells in peripheral blood and cord blood by flow cytometry. Exp Hematol 22:1003–1010

    PubMed  CAS  Google Scholar 

  37. Gratama JW, Orfao A, Barnett D, Brando B, Huber A, Janossy G, Johnsen HE, Keeney M, Marti GE, Preijers F, Rothe G, Serke S, Sutherland DR, Van der Schoot CE, Schmitz G, Papa S (1998) Flow cytometric enumeration of CD34(+) hemat-opoietic stem and progenitor cells. Cytometry 34:128–142

    Article  PubMed  CAS  Google Scholar 

  38. Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I (1996) The ISHAGE guidelines for CD34 cell determination by flow cytometry. J Hematother 5:213–226

    Article  PubMed  CAS  Google Scholar 

  39. Barnett D, Granger V, Kraan J, Whitby L, Reilly JT, Papa S, Gratama JW (2000) Reduction of intra and interlaboratory variation in CD34 positive stem cell enumeration by the use of stable test material, standard protocols and targeted training. Br J Haematol 108:784–792

    Article  PubMed  CAS  Google Scholar 

  40. Goddard G, Martin JC, Naivar M, Goodwin PM, Graves SW, Habbersett R, Nolan JP, Jett JH (2006) Single particle high resolution spectral analysis flow cytometer. Cytometry 69A:842–851

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Pharmacology Research, CDER, White Oak, MD

    James L. Weaver

  2. Laboratory of Pathology, NCI, NIH, Bethesda, MD

    Maryalice Stetler-Stevenson

Authors
  1. James L. Weaver
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maryalice Stetler-Stevenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Hertfordshire, Hatfield, Hertfordshire, UK

    John M. Walker (Professor Emeritus) & Ralph Rapley (Professor Emeritus) & 

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Weaver, J.L., Stetler-Stevenson, M. (2008). Biomedical Uses of Flow Cytometry. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_57

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-375-6_57

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-370-1

  • Online ISBN: 978-1-60327-375-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation