Skip to main content

Mammalian Cell Culture

  • Protocol
  • 4591 Accesses

Part of the book series: Springer Protocols Handbooks ((SPH))

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Harrison RG (1907) Observations on the living developing nerve fiber. Proc Soc Exp Biol Med 4:140–143

    Google Scholar 

  2. Earle WR, Schilling EL, Stark TH, Straus NP, Brown MF, Shelton E (1943) Production of malignancy in vitro I V. The mouse fibroblast cultures and changes seen in the living cells. J Natl Cancer Inst 4:165–212

    CAS  Google Scholar 

  3. Gey GO, Coffman WD, Kubicek MT (1952) Tissue culture studies of the pro-liferative capacity of cervical carcinoma and normal epithelium. Cancer Res 12:364–365

    Google Scholar 

  4. Masters JR (2002) HeLa cells 50 years on: the good, the bad and the ugly. Nature Rev 2:315–318

    Article  CAS  Google Scholar 

  5. Weaver VM, Fischer AH, Peterson OW, Bissel MJ (1996) The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. Biochem Cell Biol 74:833–851

    Article  PubMed  CAS  Google Scholar 

  6. Weaver VM, Bissel MJ (1999) Functional culture models to study mechanisms governing apoptosis in normal and malignant mammary epithelial cells. J Mammary Gland Biol Neopl 4:193–201

    Article  CAS  Google Scholar 

  7. Eagle H (1955) Amino acid metabolism in mammalian cultures. Science 130:432–437

    Article  Google Scholar 

  8. Eagle H (1955) Nutrition needs of mammalian cells in culture. Science 122:501

    Article  PubMed  CAS  Google Scholar 

  9. Dulbecco R, Freeman G (1959) Plaque formation by polyoma virus Virology 8:396–397

    Article  PubMed  CAS  Google Scholar 

  10. Iscove NN, Guilbert LW, Weyman C (1980) Complete replacement of serum in primary culture of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferring, iron, unsaturated fatty acid, lecithin and cholesterol. Exp Cell Res 126:121–126

    Article  PubMed  CAS  Google Scholar 

  11. Morgan JF, Morton HJ, Parker RC (1950) The nutrition of animal cells in tissue culture. Initial studies on a synthetic medium. Proc Soc Exp Biol Med 73:1–8

    PubMed  CAS  Google Scholar 

  12. Parker RC (1957) Altered cell strains in continuous culture: a general survey. In: Whitelock O (ed) Special Publications of the New York Academy of Sciences: 5, NY Acad Sci., New York, pp 303–313

    Google Scholar 

  13. Waymouth C (1959) Proliferation of sublines of NCTC clone 929 (strain L0 mouse cells in a simple chemically defined medium (MB752/1). J Natl Cancer Inst 22:1003

    PubMed  CAS  Google Scholar 

  14. Ham RG (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp Cell Res 29:515–526

    Article  PubMed  CAS  Google Scholar 

  15. Ham RG (1965) Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA 53:288–293

    Article  PubMed  CAS  Google Scholar 

  16. McCoy TA, Maxwell M, Kruse PF (1959) Amino acid requirements of the Novikoff hepatoma in vitro. Proc Soc Exp Biol Med 100:115–118

    PubMed  CAS  Google Scholar 

  17. Moore GE, Gerner RE, Franklin HA (1967) Culture of normal human leukocytes. JAMA 199:519–524

    Article  PubMed  CAS  Google Scholar 

  18. Barnes D, Sato G (1980) Serum-free culture: a unifying approach. Cell 22:649–655

    Article  PubMed  CAS  Google Scholar 

  19. Barnes D, Sato G (1980) Methods for growth of cultured cells in serum-free medium. Anal Biochem 102:519–524

    Article  Google Scholar 

  20. Ham RG, McKeehan WL (1978) Development of improved media and culture conditions for clonal growth of normal diploid cells. In vitro 14:11–22

    Article  PubMed  CAS  Google Scholar 

  21. Hayflick L, Moorhead PS (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25:585–621

    Article  Google Scholar 

  22. Nelson-Rees WA, Daniels DW, Flandermeyer RR (1981) Cross-contamination of cells in culture. Science 212:446–452

    Article  PubMed  CAS  Google Scholar 

  23. Boyd MR, Paull KD (1995) Some practical considerations and applications of the National Cancer Institute In Vitro Drug Discovery Screen. Drug Development Research 34:91–109

    Article  CAS  Google Scholar 

  24. Dieterich C, Schandar M, Noll M, Johannes F, Brunner H, Graeve T, Rupp (2002) In vitro reconstructed human epithelia reveal contributions of Candida albicans EFG1 and CPH1 to adhesion and invasion. Microbiology 148:497–506

    PubMed  CAS  Google Scholar 

  25. Lawrence S (2005) Biotech drug market steadily expands. Nature Biotechnol 23:1466

    Article  CAS  Google Scholar 

  26. Pavlou A, Belsy M (2005) The therapeutic antibody market to 2008. Eur J Pharm Biopharm 59:389–396

    Article  PubMed  Google Scholar 

  27. Pavlou A, Reichert J (2004) Recombinant protein therapeutics – success rates, market trends and values to 2010. Nature Biotechnol 22:1513–1519

    Article  CAS  Google Scholar 

  28. Wurm FW (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotech 22:1393–1398

    Article  CAS  Google Scholar 

  29. Walsh G (2006) Biopharmaceutical benchmarks 2006. Nature Biotechnol 24:769–776

    Article  CAS  Google Scholar 

  30. Walsh G (2003) Biopharmaceutical benchmarks 2003. Nature Biotechnol 21:865–870

    Article  CAS  Google Scholar 

  31. Ott MG, Schmidt M, Schwarzaelder K, Stein S, Siler U, Koehl U (2006) Correction of X-linked chronic granulomatous disease by gene therapy, augmented by inser-tional activation of MDS1-EVI1, PRDM16 or SETBP1. Nature Med 12:401–409

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Langdon, S.P. (2008). Mammalian Cell Culture. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_49

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-375-6_49

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-370-1

  • Online ISBN: 978-1-60327-375-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics