Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Smith HO, Wilcox KW (1970) A restriction enzyme from Haemophilus influenzae: purification and general characterization. J Mol Biol 51:379
Jones P, Qiu J, Rickwood D (1994) RNA isolation and analysis. Bios Scientific. Oxford
Heptinstall J (2000) Spectrophotometric analysis of nucleic acids. In: Rapley R (ed) The nucleic acid protocols handbook. Humana, Totowa, NJ
Bryant S, Manning DL (2000) Isolation of mRNA by affinity chromatography. In: Rapley R (ed) The nucleic acid protocols handbook. Humana, Totowa, NJ
Maule J (2000) Pulsed-field gel electrophoresis. In: Rapley R (ed) The nucleic acid protocols handbook. Humana, Totowa, NJ
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503
Alwine JC, Kemp DJ, Stark GR (1977) Method for the detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74:5350
Aquino de Muro M, Rapley R (2002) Gene probes: principles and protocols. Humana, Totowa, NJ
Zamore PD, Tuschi T, Sharp PA, Bartel DP (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRMA at 21 to 23 nucleotide intervals. Cell 101 (1):25–33
Nykanen A, Haley B, Zamore PD (2001) ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107 (3):309–321
Bitko V, Musiyenko A, Shulyayera O, Barik S (2005) Inhibition of respiratory viruses by nasally administered siRNA. Nat Med 11:50–55
Gonzalez-Alegre P (2007) Therapeutic RNA interference for neurodegenrative diseases: from promise to progress. Pharmacol Therap Epub ahead of print
Andersen ND, Monahan TS, Malek JY, Jain M, Daniel S, Caron LD, Pradhan L, Ferran C, LoGerto FW (2007) Comparison of gene silencing in human vascular cells using small interfering RNAs. J Am Col Surg 204:399–408
Masiero M, Nardo G, Indraccolo S, Favaro, E (2007) RNA interference: implications for cancer treatment. Mol Aspects Med 28:143–166
Bumcrot D, Manoharan M, Koteliansky V, Sah DWY (2006) RNAi therapeutics: a potential new class of pharmacological drugs. Nat Chem Biol 2 (12):711–719
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Corkill, G., Rapley, R. (2008). The Manipulation of Nucleic Acids. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_1
Download citation
DOI: https://doi.org/10.1007/978-1-60327-375-6_1
Publisher Name: Humana Press
Print ISBN: 978-1-60327-370-1
Online ISBN: 978-1-60327-375-6
eBook Packages: Springer Protocols