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Liposomal siRNA Delivery

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Liposomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 605))

Abstract

With the recent discovery of small interfering RNA (siRNA), to silence the expression of genes in vitro and in vivo, there has been a need to deliver these molecules to the cell nucleus. Forming a lipid/nucleic acid complex has become a solution and is explored here. Certain methods and ideas are used, such as: the positive/negative electrostatic interaction with a cationic lipid and an anionic RNA molecule, the size of the lipid vesicle aiding the uptake target tissues, targeted lipoplexes which can increase efficiency, and the protection of the siRNA molecule from the natural defenses of the immune system. Many lipid formulations exist and can be experimented with to achieve varying results depending on the application.

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Authors and Affiliations

  1. Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA

    Jeffrey Hughes, Preeti Yadava & Ryan Mesaros

Authors
  1. Jeffrey Hughes
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  2. Preeti Yadava
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  3. Ryan Mesaros
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Corresponding author

Correspondence to Jeffrey Hughes .

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Editors and Affiliations

  1. College of Pharmacy, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, U.S.A.

    Volkmar Weissig

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Hughes, J., Yadava, P., Mesaros, R. (2010). Liposomal siRNA Delivery. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology, vol 605. Humana Press. https://doi.org/10.1007/978-1-60327-360-2_31

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  • DOI: https://doi.org/10.1007/978-1-60327-360-2_31

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-359-6

  • Online ISBN: 978-1-60327-360-2

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