Abstract
Helicases are ubiquitous enzymes that are vital to all living organisms. They are motor proteins that move in a specific direction along the nucleic acid and unwind the nucleic acid (DNA and RNA). ATP hydrolysis provides energy for helicase translocation and unwinding. The unwinding process provides ssDNA intermediates necessary for replication, recombination, and repair. Mutations in specific DNA helicases can lead to disruption in DNA metabolism. For example, mutations in helicases genes resulted in diseases such as xeroderma pigmentosum, cockayne’s syndrome, Bloom’s syndrome, and Werner’s syndrome. During unwinding, helicases are most likely to encounter proteins while moving along the nucleic acid. Several different research groups have demonstrated that helicases shift or displace proteins from one nucleic acid-bound location to another. These protein–protein collisions could result in displacement of proteins from nucleic acid or dissociation of helicase from nucleic acid. This report describes several different methods developed to study protein displacement by DNA and RNA helicases.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Yeruva, L., Raney, K.D. (2009). Protein Displacement by Helicases. In: Abdelhaleem, M. (eds) Helicases. Methods in Molecular Biology, vol 587. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-355-8_6
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DOI: https://doi.org/10.1007/978-1-60327-355-8_6
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