Abstract
In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.
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References
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Acknowledgments
The authors would like to thank Jaya Yodh for carefully reading this chapter and providing helpful comments. The authors thank Jaya Yodh, Salman Sayed, Jeehae Park, and Hamza Balci for providing data, comments, and manuscripts in preparation. E.R. is a fellow of the NSF Center for the Physics of Live Cells at the University of Illinois, and he acknowledges its support.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Rothenberg, E., Ha, T. (2009). Single-Molecule FRET Analysis of Helicase Functions. In: Abdelhaleem, M. (eds) Helicases. Methods in Molecular Biology, vol 587. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-355-8_3
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DOI: https://doi.org/10.1007/978-1-60327-355-8_3
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60327-355-8
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