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Detection of Bartonella spp. DNA in Clinical Specimens Using an Internally Controlled Real-Time PCR Assay

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 943))

Abstract

Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a ­self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid, sensitive, and simple detection of Bartonella spp. in samples from CSD or endocarditis suspects, and it is suitable for implementation in the diagnostic microbiology laboratory.

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Author information

Authors and Affiliations

  1. Laboratory of Medical Microbiology, Franciscus Hospital, Roosendaal, The Netherlands

    Anneke M. C. Bergmans

  2. Laboratory of Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands

    Anneke M. C. Bergmans

  3. Laboratory of Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands

    John W. A. Rossen

Authors
  1. Anneke M. C. Bergmans
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  2. John W. A. Rossen
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Corresponding author

Correspondence to Anneke M. C. Bergmans .

Editor information

Editors and Affiliations

  1. Deptartment of Microbiology, St. Bartholomew's Hospital, Newark Street 80, London, E1 2ES, United Kingdom

    Mark Wilks

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Bergmans, A.M.C., Rossen, J.W.A. (2013). Detection of Bartonella spp. DNA in Clinical Specimens Using an Internally Controlled Real-Time PCR Assay. In: Wilks, M. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology, vol 943. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-353-4_14

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  • DOI: https://doi.org/10.1007/978-1-60327-353-4_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-352-7

  • Online ISBN: 978-1-60327-353-4

  • eBook Packages: Springer Protocols

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