Retinoids pp 295-313 | Cite as

Diet in Vitamin A Research

  • A. Catharine Ross
Part of the Methods in Molecular Biology book series (MIMB, volume 652)


A properly formulated diet is an essential underpinning for all in vivo research. This chapter focuses on the use of diet in retinoid research from two perspectives: human research, in which diet is usually variable and analysis of dietary intake is paramount to interpreting the study’s results, and animal (rodent) research, in which diet is imposed as a factor in the experimental design, and the diet consumed is usually monotonous. Many standard rodent diets are nonpurified and the amount of vitamin A in the diet is unknown. Moreover, it is likely to be much higher than expected from the label. By using a well-formulated purified diet with an exact amount of vitamin A, retinoid status in rodents can be closely controlled to create specific physiological conditions that represent the wide range of vitamin A status present in human populations.

Key words

Diet dietary assessment retinol retinoic acid vitamin A deficiency vitamin A supplementation 



American Institute of Nutrition


Institute of Medicine


National Research Council


retinoic acid


recommended dietary allowance.



Support: NIH grants CA-90214; DK-41479.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • A. Catharine Ross
    • 1
  1. 1.Department of Nutritional Sciences and Huck Institute for the Life SciencesPennsylvania State UniversityUniversity ParkUSA

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