High-Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden
Bioluminescence imaging (BLI) has emerged during the past 5 years as the preeminent method for rapid, cheap, facile screening of tumor growth and spread in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing literature on routine use of BLI for assessing chemotherapeutic efficacy, drug combinations, dosing, and timing. In addition, BLI may be applied to more sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.
Key wordsLuciferase luciferin charged-coupled device cameras (CCD) IGOR Pro bioluminescence
Supported in part by grants from the DOD Breast Cancer Initiative (IDEA award DAMD17-03-1-0343), the NIH Cancer Imaging Program (P20 CA86354 and U24 CA126608), and the Simmons Cancer Center. We are grateful to Drs. Li Liu, Robert Bollinger, Jerry Shay, and Peter Antich for bringing the vision of BLI to UT Southwestern.
- 8.Bollinger, R. A. (2006), Ph.D., UT Southwestern, Dallas.Google Scholar
- 10.Cecic, I., Chan, D. A., Sutphin, P., Ray, P., Gambhir, S. S., Giaccia, A. J., and Graves, E. E. (2007) Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia. Mol Imaging Biol 6, 219–228.Google Scholar
- 11.Karam, J. A., Fan, J., Stanfield, J., Richer, E., Benaim, E. A., Frenkel, E., Antich, P., Sagalowsky, A. I., Mason, R. P., and Hsieh, J. -T. (2007) The use of histone deacetylase inhibitor FK228 and DNA hypomethylation agent 5-Azacytidine in human bladder cancer therapy. Int J Cancer 120, 1795–1802.PubMedCrossRefGoogle Scholar
- 14.Kanto, V., Munger, J., and Berry, R. (1994) The CCD Camera Cookbook, Willman-Bell, Inc, Richmond, VA.Google Scholar
- 15.Dikmen, Z. G., Gellert, G., Dogan, P., Mason, R., Antich, P., Richer, E., Wright, W. E., and Shay, J. E. (2005) A new diagnostic system in cancer research: bioluminescent imaging (BLI). Turk J Med Sci 35, 65–70.Google Scholar