Abstract
Plasma membranes encompass a complex and varying set of proteins essential to life. In addition, plasma membrane proteins represent the majority of all known drug targets. The characterization of plasma membrane proteomes is, therefore, of eminent importance. A current bottleneck is the lack of efficient protocols to isolate plasma membranes from tissues or entire organs. To this end, we recently established a simple and effective isolation procedure which is based on aqueous polymer two-phase systems. In this chapter, we provide a detailed protocol for the isolation of plasma membranes from brain tissue, which could easily be adapted to other sources.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Hopkins AL, Groom CR (2002) The druggable genome. Nat Rev Drug Discov 1, 727–730.
Albertsson P-A (1971) Binding Studies. In: Partition of cell particles and macromolecules (Albertsson P.-A., ed), pp. 233–242. New York: Wiley-Interscience.
Gierow P, Sommarin M, Larsson C, Jergil B (1986) Fractionation of rat liver plasma-membrane regions by two-phase partitioning. Biochem J 235, 685–691.
Persson A, Johansson B, Olsson H, Jergil B (1991) Purification of rat liver plasma membranes by wheat-germ-agglutinin affinity partitioning. Biochem J 273, 173–177.
Persson A, Jergil B (1995) The purification of membranes by affinity partitioning. FASEB J 9, 1304–1310.
Schindler J, Lewandrowski U, Sickmann A, Friauf E, Gerd NH (2006) Proteomic analysis of brain plasma membranes isolated by affinity two-phase partitioning. Mol Cell Proteomics 5, 390–400.
Aronson NN, Jr., Touster O (1974) Isolation of rat liver plasma membrane fragments in isotonic sucrose. Methods Enzymol 31, 90–102.
Fujiki Y, Hubbard AL, Fowler S, Lazarow PB (1982) Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol 93, 97–102.
Taylor RS, Wu CC, Hays LG, Eng JK, Yates JR, Howell KE (2000) Proteomics of rat liver Golgi complex: minor proteins are identified through sequential fractionation. Electrophoresis 21, 3441–3459.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Schindler, J., Nothwang, H.G. (2009). Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning. In: Peirce, M.J., Wait, R. (eds) Membrane Proteomics. Methods in Molecular Biology™, vol 528. Humana Press. https://doi.org/10.1007/978-1-60327-310-7_8
Download citation
DOI: https://doi.org/10.1007/978-1-60327-310-7_8
Publisher Name: Humana Press
Print ISBN: 978-1-60327-309-1
Online ISBN: 978-1-60327-310-7
eBook Packages: Springer Protocols