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Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ In Vitro

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Mouse Molecular Embryology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1092))

Abstract

A well-established experimental paradigm to analyze gene function in development is to elucidate the impact of gain and loss of gene activity on cell differentiation, tissue modelling, organogenesis, and morphogenesis. This chapter describes the experimental protocols to study gene function by means of electroporation and lipofection to manipulate genetic activity in whole embryos and fetal organs in vitro. These techniques allow for more precise control of the timing, with reference to developmental age or stage, and the cell/tissue-specificity of the changes in gene activity. They provide an alternative strategy that can expedite the analysis of gene function before resorting to the conventional means of transgenesis and gene targeting in the whole organism.

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Author information

Authors and Affiliations

  1. Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan

    Satomi S. Tanaka & Yasuka L. Yamaguchi

  2. Children’s Medical Research Institute, Sydney Medical School, The University of Sydney, Sydney, Australia

    Vanessa J. Jones & Patrick P. L. Tam

Authors
  1. Satomi S. Tanaka
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  2. Yasuka L. Yamaguchi
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  3. Vanessa J. Jones
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  4. Patrick P. L. Tam
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Editor information

Editors and Affiliations

  1. Center, National Cancer Institute Frederick Cancer Research & Development, Frederick, Maryland, USA

    Mark Lewandoski

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© 2014 Springer Science+Business Media, New York

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Tanaka, S.S., Yamaguchi, Y.L., Jones, V.J., Tam, P.P.L. (2014). Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ In Vitro. In: Lewandoski, M. (eds) Mouse Molecular Embryology. Methods in Molecular Biology, vol 1092. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-60327-292-6_22

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  • DOI: https://doi.org/10.1007/978-1-60327-292-6_22

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  • Publisher Name: Humana Press, Boston, MA

  • Print ISBN: 978-1-60327-290-2

  • Online ISBN: 978-1-60327-292-6

  • eBook Packages: Springer Protocols

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