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Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study

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Mouse Molecular Embryology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1092))

Abstract

The need for large-scale collection of rodent embryos and individual embryonic tissues for genomic and proteomic studies requires modification of traditional practices of embryo necropsy. The sample intended for transcriptome study should be rapidly dissected and stabilized to preserve its molecular integrity. The retrieval of high-quality RNA, DNA, and proteins from the target tissue is crucial for informative molecular analysis (e.g., gene profiling on microarray platform). We present a reliable method of collection and preparation of rodent embryos for genomic studies supported by detailed protocols and RNA extraction results for different stages of mouse embryonic development.

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Acknowledgments

The authors would like to thank Anna Trivet of NCI-Frederick for her technical expertise and Keith Rogers, currently of BSF–Singapore for his continuous support during many different phases of this study, also Scott Lawrence of NCI-FCRDC, DCTD, DTP, PADIS for generous help with photography and Tamara Morgan of Histotechnology Laboratory, NCI–Frederick for technical assistance. This work was funded by NCI Contract HHSN261200800001E.

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Authors and Affiliations

  1. Histotechnology Laboratory, SAIC, Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA

    Yelena Golubeva

  2. Departments of Molecular Virology, Immunology and Medical Genetics, Internal Medicine, and Biomedical Informatics, Ohio State University Comprehensive Cancer Center, Columbus, OH, USA

    David Symer

Authors
  1. Yelena Golubeva
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  2. David Symer
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Editors and Affiliations

  1. Center, National Cancer Institute Frederick Cancer Research & Development, Frederick, Maryland, USA

    Mark Lewandoski

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© 2014 Springer Science+Business Media, New York

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Golubeva, Y., Symer, D. (2014). Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study. In: Lewandoski, M. (eds) Mouse Molecular Embryology. Methods in Molecular Biology, vol 1092. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-60327-292-6_20

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  • DOI: https://doi.org/10.1007/978-1-60327-292-6_20

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  • Publisher Name: Humana Press, Boston, MA

  • Print ISBN: 978-1-60327-290-2

  • Online ISBN: 978-1-60327-292-6

  • eBook Packages: Springer Protocols

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