Summary
Medicinal plants are sources of important therapeutic aid for healing human diseases. The depletion of the wild resources has prompted conservation, propagation, and enhancement of resources for medicinal plants. Micropropagation offers an alternate method to propagate and improve medicinal plants through selection of high-yield lines and their efficient cloning. This chapter describes cost effective and efficient protocols that have been successfully applied for the micropropagation and large-scale production of quality planting material in two important tuberous medicinal plants viz., Holostemma ada-kodien Schult. and Ipomoea mauritiana Jacq.
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References
Anonymous (1959). The Wealth of India – A Dictionary of Indian Raw Materials and Industrial Products. Raw Materials Vol. V (H–K), Council of Scientific and Industrial Research (CSIR), New Delhi, India, p. 111
Anonymous (2002). The Wealth of India – A Dictionary of Indian Raw Materials and Industrial Products. Raw Materials Vol. 3 (D–I), National Institute of Science Communication (NISCOM), CSIR, New Delhi, India, p. 294
Sivarajan, V. V. and Balachandran, I. (1994). Ayurvedic Drugs and their Plant Sources, Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India, pp. 195–196, 510–511
Rastogi, P.R., Mehrotra, B.N., and Srivastava, M. (1993). Compendium of Indian medicinal plants. Vol: 3. Central Drug Research Institute, Lucknow, India, pp. 346–347
Rastogi, P.R., Mehrotra, B.N., and Srivastava, M. (1990). Compendium of Indian medicinal plants. Vol: 1. Central Drug Research Institute, Lucknow, India, pp. 226–227
Rastogi, P.R., Mehrotra, B.N., and Srivastava, M. (1991). Compendium of Indian medicinal plants. Vol: 3. Central Drug Research Institute, Lucknow, India, pp. 392
Martin, K. P. (2002). Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Rep. 21(2): 112–117
Martin, K. P. (2003). Plant regeneration through somatic embryogenesis on Holostemma ada-kodien, a rare medicinal plant. Plant Cell Tissue Organ Cult. 72(1): 79–82
Sudha, C.G., Krishnan, P.N. and Pushpangadan, P. (1998). In vitro propagation of Holostemma annulare. K. Schum, a rare medicinal plant. In Vitro Cell. Dev. Biol. 20(4): 34–57
Sudha, C.G., Krishnan, P.N. and Pushpangadan, P. (2002). Regeneration of plants from in vitro root segments of Holostemma annulare. Schum – a rare medicinal plant. Curr. Sci. 78: 503
Geetha S.P., Indira Balachandran and P. N. Ravindran (2007). In vitro shoot culture and micro-tuber induction in Jivanti (Holostemma ada-kodien Schult.). In: Proceedings National Symposium on ‘Plant Biotechnology: New Frontiers’, Kukreja AK et al. (eds) 18–20 November, 2005, Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India, pp. 202–206
Priya, L., Anita, S., Rajmohan, K., and Alex, S. (2007). In vitro shoot regeneration in Ipomoea mauritiana Linn. In: Kesavachandran, R., Nazeem, P. A., Girija, D., John, P. S., and Peter, K. V. (eds.). Recent Trends in Horticultural Biotechnology, New India Publishing Agency, New Delhi, India, pp. 291–293
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Acknowledgments
The authors would like to thank Sir Dorabji TATA Trust, Mumbai, India for the financial support.
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Geetha, S.P., Raghu, A.V., Martin, G., George, S., Balachandran, I. (2009). In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana . In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_7
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DOI: https://doi.org/10.1007/978-1-60327-287-2_7
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