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Micropropagation of Penthorum chinenseThrough Axillary Buds

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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 547))

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Summary

Penthorum chinensePursh is a traditional medicinal herb in China. Micropropagation protocol of this plant has been established. The shoot induction rate is high by culturing nodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) as compared with kinetin (Kn). After 6 wks, the highest shoot formation (5.2) is achieved in 59.2% nodal explants cultured on MS medium combined with 2.0 mg/L BA. After 4 wk of subculture on the fresh MS medium, the highest shoot multiplication rate 6.4 is accomplished. MS medium containing 1.0 mg/L BA is most suitable for shoot propagation. Individual well developed shoots (0.8-to 1.0-cm long) are excised and culture on the MS medium containing with 0.5 mg/L indole-3-acetic acid (IAA), for rooting. Three weeks later, 98.8% of shoots had rooted with an average of ten roots per shoot. Plantlets transferred to soil were successfully acclimatized. This protocol will facilitate the conservation and propagation of this important medicinal plant.

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Acknowledgments

This work is financially assisted by Sichuan Provincial Youth Science and Technology Foundation (05ZQ026-017), and Sichuan Provincial Key Subject Program (SZD0420), P. R. China.

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Authors and Affiliations

  1. College of Life Science, China West Normal University, Nanchong, Sichuan, 637002, P. R., China

    Jun Yang & Zheng-song Peng

Authors
  1. Jun Yang
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  2. Zheng-song Peng
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Corresponding author

Correspondence to Jun Yang .

Editor information

Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Praveen K. Saxena

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Yang, J., Peng, Zs. (2009). Micropropagation of Penthorum chinenseThrough Axillary Buds. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_16

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  • DOI: https://doi.org/10.1007/978-1-60327-287-2_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-286-5

  • Online ISBN: 978-1-60327-287-2

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