Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants pp 129-138 | Cite as
Micropropagation and In Vitro Conservation of Vanilla (Vanilla planifolia Andrews)
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Summary
Vanilla (Vanilla planifolia Andrews (syn. V. fragrans Salisb.), a source of natural vanillin, plays a major positive role in the economy of several countries. A native to the Central America, its primary gene pool is threatened by deforestation and over collection that has resulted in disappearance of natural habitats and wild species. Therefore, multiplication and conservation of vanilla diversity is of paramount importance because of its narrow genetic base. It plays an important role in the production of disease free planting material for commercial cultivation. Simple protocols for micropropagation, in vitro conservation and synthetic seed production are described in this chapter which could further be applied to other related vanilla species as well.
Key words
Embryo rescue In vitro conservation Micropropagation Plant regeneration Seed culture Somaclones Synthetic seeds VanillaReferences
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