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Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity

  • Mirjana Macvanin
  • Diarmaid Hughes
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 642)

Abstract

Bacteria, in common with other organisms that take advantage of aerobic respiration, generate and accumulate reactive oxygen species (ROS) that damage DNA, fatty acids, and proteins. In addition, intracellular pathogens like Salmonella enterica are exposed to an oxidate burst produced by host macrophages. The relative ability of aerobically growing bacteria to withstand oxidative stress and eliminate ROS has a large impact of their fitness in vitro and in vivo. Methods are described here to measure the viability and relative fitness of bacteria in the presence of hydrogen peroxide. A protocol for the determination of catalase activity, an important part of the ROS detoxification process, is also described.

Key words

Antibiotic resistance Fitness cost Reactive oxygen species Hydrogen peroxide Catalase Salmonella typhimurium 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Mirjana Macvanin
    • 1
  • Diarmaid Hughes
    • 2
  1. 1.Laboratory of Molecular Biology, National Cancer InstituteNational Institutes of HealthBethesdaUSA
  2. 2.Microbiology Programme, Department of Cell and Molecular BiologyUppsala UniversityUppsalaSweden

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