Abstract
It is often desirable to know how much of a particular protein is produced by a particular cell. For example, is this protein expressed at low levels throughout a population of cells, or is this protein expressed at very high levels in some cells and not in others? Does the expression of the protein change in a particular cell-cycle compartment? Technical protocols that require cell lysis, such as immunoblotting (1), allow the mean expression level of a given protein to be compared between populations of cells. It is even possible to use the immunoblotting protocol to determine the amount of a given protein expressed by a population of cells, if the protein has been purified for use as a standard. However, cell lysis obliterates the potential for single-cell quantitation of proteins.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Ray, F.A., Friedrich, T.D., Laffin, J., Lehman, J.M. (1996). Protein Quantitation Using Flow Cytometry. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_7
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DOI: https://doi.org/10.1007/978-1-60327-259-9_7
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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