Abstract
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Chial, H. J., Thompson, H. B., and Splittgerber, A. G. (1993) A spectral study of the charge forms of Coomassie blue G. Anal. Biochem. 209, 258–266.
Compton, S. J. and Jones, C. G (1985) Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem. 151, 369–374.
Congdon, R. W., Muth, G. W., and Splittgerber, A. G. (1993) The binding interaction of Coomassie blue with proteins. Anal. Biochem. 213, 407–413.
Friendenauer, S. and Berlet, H. H. (1989) Sensitivity and variability of the Bradford protein assay in the presence of detergents. Anal. Biochem. 178, 263–268.
Reade, S. M. and Northcote, D. H. (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal. Biochem. 116, 53–64.
Stoscheck, C. M. (1990) Increased uniformity in the response of the Coomassie blue protein assay to different proteins. Anal. Biochem. 184, 111–116.
Spector, T. (1978) Refinement of the Coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for <0.5 to 50 µg of protein. Anal. Biochem. 86, 142–146.
Peterson, G. L. (1983) Coomassie blue dye binding protein quantitation method, in Methods in Enzymology, vol. 91 (Hirs, C. H. W. and Timasheff, S. N., eds.), Academic, New York, pp. 95–119.
Kirazov, L. P., Venkov, L. G., and Kirazov, E. P. (1993) Comparison of the Lowry and the Bradford protein assays as applied for protein estimation of membrane-containing fractions. Anal. Biochem. 208, 44–48.
Wilson, C. M. (1979) Studies and critique of Amido Black 10B, Coomassie blue R and Fast green FCF as stains for proteins after polyacrylamide gel electrophoresis. Anal. Biochem. 96, 263–278.
Sedmak, J. J. and Grossberg, S. E. (1977) A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Anal. Biochem. 79, 544–552.
Redinbaugh, M. G. and Campbell, W. H. (1985) Adaptation of the dye-binding protein assay to microtiter plates. Anal. Biochem. 147, 144–147.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Kruger, N.J. (1996). The Bradford Method for Protein Quantitation. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_4
Download citation
DOI: https://doi.org/10.1007/978-1-60327-259-9_4
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
eBook Packages: Springer Book Archive