This chapter examines the aspects of using ELISA to solve problems, definitions of terms met in serology, antibody structure, and the production of antibodies in animals, units, dilutions, and molarities. Antibodies — A Laboratory Manual (1) is an excellent manual of techniques relevant to ELISA, and all scientists involved in experimental work involving antibodies should have this manual. The manuals given in refs. 2 and 3 also provide extensive relevant practical information.
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References
Harlow, E., and Lane, D., eds. (1988) Antibodies — A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Catty, D., ed. (1988) Antibodies — A Practical Approach, Volume I, IRL Press, Oxford, England .
Catty, D., ed. (1988) Antibodies – A Practical Approach, Volume II, IRL Press, Oxford, England .
Linscott, W.D. (1982)Linscott's Director y of Immunological and Biological Reagents, Linscott's Catalog, Mill Valley, CA.
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(2009). Theoretical Considerations. In: The ELISA Guidebook. Methods in Molecular Biology™, vol 516. Humana Press. https://doi.org/10.1007/978-1-60327-254-4_5
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DOI: https://doi.org/10.1007/978-1-60327-254-4_5
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