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Solution-State Nuclear Magnetic Resonance Spectroscopy and Protein Folding

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Protein Folding, Misfolding, and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 752))

Abstract

A protein undergoes a variety of structural changes during its folding and misfolding and a knowledge of its behaviour is key to understanding the molecular details of these events. Solution-state NMR spectroscopy is unique in that it can provide both structural and dynamical information at both high-resolution and at a residue-specific level, and is particularly useful in the study of dynamic systems. In this chapter, we describe NMR strategies and how they are applied in the study of protein folding and misfolding.

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Acknowledgements

We would like to thank the members of the Christodoulou lab for useful discussions and Dr. John Kirkpatrick for critical reading of this chapter.

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Authors and Affiliations

  1. Department of Structural and Molecular Biology, University College London, London, UK

    John Christodoulou

  2. School of Crystallography, Birkbeck College, London, UK

    John Christodoulou

Authors
  1. Lisa D. Cabrita
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  2. Christopher A. Waudby
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  3. Christopher M. Dobson
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  4. John Christodoulou
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Corresponding author

Correspondence to John Christodoulou .

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Editors and Affiliations

  1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Andrew F. Hill

  2. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Kevin J. Barnham

  3. , Department of Biochemistry &, Monash University, Wellington Road, Clayton, 3800, Victoria, Australia

    Stephen P. Bottomley

  4. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Roberto Cappai

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Cabrita, L.D., Waudby, C.A., Dobson, C.M., Christodoulou, J. (2011). Solution-State Nuclear Magnetic Resonance Spectroscopy and Protein Folding. In: Hill, A., Barnham, K., Bottomley, S., Cappai, R. (eds) Protein Folding, Misfolding, and Disease. Methods in Molecular Biology, vol 752. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-223-0_7

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  • DOI: https://doi.org/10.1007/978-1-60327-223-0_7

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