Abstract
This chapter describes method for evaluation of nanomaterial cytotoxicity by examining effects on porcine kidney (LLC-PK1) and human cancerous liver cells (Hep G2). Several studies indicate that many nanoparticles are cleared from the body through the kidney or liver, making these organs good choices for target organ toxicity evaluation. In this standard, two separate metrics (MTT and LDH) provide complementary data, that can be used to identify interference.
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References
Alley, M.C., Scudiero, D.A., Monks, A. et al. (1998) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48, 589–601.
Slater, T.F., Sawyer, B. and Struli, U. (1963) Studies on succinate-tetrazolium reductase systems III, points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta 77, 383–393.
Altman, F.P. (1976) Tetrazolium salts and formazans. Prog. Histochem. Cytochem. 9, 1–56.
Burdon, R.H., Gill, V. and Rice-Evens, C. (1993) Reduction of a tetraxolium salt and superoxide generation in human tumor cells (HeLa). Free Radic. Res. Commun. 18, 369–380.
Liu, Y., Peterson, D.A., Kimura, H. and Schubert, D. (1997) Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. J. Neurochem. 69, 581–593.
Decker, T. and Lohmann-Matthes, M.L. (1988) A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J. Immunol. Methods 115, 61–69.
Korzeniewski, C. and Callewaert, D.M. (1983) An enzyme-release assay for natural cytotoxicity. J. Immunol. Methods 64, 313–320.
Acknowledgments
This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
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Potter, T.M., Stern, S.T. (2011). Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_16
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DOI: https://doi.org/10.1007/978-1-60327-198-1_16
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Print ISBN: 978-1-60327-197-4
Online ISBN: 978-1-60327-198-1
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