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Methods and Algorithms for Relative Quantitative Proteomics by Mass Spectrometry

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Bioinformatics Methods in Clinical Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 593))

Abstract

Protein quantitation by mass spectrometry (MS) is attractive since it is possible to obtain both the identification and quantitative values of novel proteins and their posttranslational modifications in one experiment. In contrast, protein arrays only provide quantitative values of targeted proteins and their modifications. There are an overwhelming number of quantitative mass spectrometry (MS) methods for protein and peptide quantitation. The aim here is to provide an overview of the most common MS-based quantitative methods used in the proteomics field and discuss the computational algorithms needed for the robust quantitation of proteins, peptides, and their posttranslational modifications.

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Acknowledgments

Support for RM was provided from Ramon y Cajal (RYC-2006-001446) and Fundação para a Ciência e a Tecnologia, Programa CIÊNCIA 2007 (C2007-IPATIMUP/AA2). Ana Sofia Carvalho gratefully thanks Fundação para a Ciência e a Tecnologia for a postdoctoral fellowship (SFRH/BPD/36912/2007).

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Authors and Affiliations

  1. Instituto de Patologia e Imunologia Molecular da Universidad do Porto – IPATIMUP, Porto, Portugal

    Rune Matthiesen & Ana Sofia Carvalho

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  1. Rune Matthiesen
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  2. Ana Sofia Carvalho
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  1. Inst. Patologia e Imunologia Molecular, Universidade do Porto, Rua Dr. Roberto Frias s/n, Porto, 4200-465, Portugal

    Rune Matthiesen

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Matthiesen, R., Carvalho, A.S. (2010). Methods and Algorithms for Relative Quantitative Proteomics by Mass Spectrometry. In: Matthiesen, R. (eds) Bioinformatics Methods in Clinical Research. Methods in Molecular Biology, vol 593. Humana Press. https://doi.org/10.1007/978-1-60327-194-3_10

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  • DOI: https://doi.org/10.1007/978-1-60327-194-3_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-193-6

  • Online ISBN: 978-1-60327-194-3

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