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Model of Neonatal Focal Cerebral Ischemia-Reperfusion

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Animal Models of Acute Neurological Injuries

Part of the book series: Springer Protocols Handbooks ((SPH))

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It has become clear that susceptibility to hypoxic-ischemic, excitotoxic, and oxidative insults is increased at specific stages of postnatal brain maturation, affecting both the acute and lasting injury patterns seen after the insult. For decades, a model of hypoxia-ischemia in postnatal days 7–9 (P7–P9) was the only rodent model relevant to ischemic brain injury in the neonate. This model is more likely to mimic global hypoxic-ischemic encephalopathy rather that focal transient ischemia of a single artery, such as the middle cerebral artery (MCA). To study the mechanisms of arterial pediatric stroke, a transient MCA occlusion model was first developed in juvenile rats. Since focal occlusion of the MCA is more commonly seen in term human babies than children and brain maturation affects the response to cerebral ischemia, we developed a transient MCA occlusion in P7 rats to satisfy the need for age-appropriate stroke models. We produced a series of models of different stroke severity by varying the duration of MCA occlusion and the age of the animals. In this chapter, we will describe in detail surgical procedures to induce MCA occlusion, ways to ascertain that the procedure is successful, and discuss factors and limitations that can affect short and longerterm injury outcomes.

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Author information

Authors and Affiliations

  1. Department of Neurology, University of California San Francisco, San Francisco, CA, USA

    Zinaida S. Vexler

  2. Department of Neurology and Pediatrics, University of California San Francisco, San Francisco, CA, USA

    Donna M. Ferriero

Authors
  1. Zinaida S. Vexler
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  2. Donna M. Ferriero
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Editor information

Editors and Affiliations

  1. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Jun Chen M.D.

  2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    Zao C. Xu M.D., Ph.D.

  3. Spinal Cord and Brain Injury Research Group Stark Neurosciences Research Institute Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN, USA

    Xiao-Ming Xu M.D., Ph.D.

  4. Department of Physiology and Pharmacology Department of Neurosurgery Department of Anesthesiology, Loma Linda University Medical School, Loma Linda, CA, USA

    John H. Zhang M.D., Ph.D.

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© 2009 Humana Press, a part of Springer Science + Business Media, LLC

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Vexler, Z.S., Ferriero, D.M. (2009). Model of Neonatal Focal Cerebral Ischemia-Reperfusion. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_20

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  • DOI: https://doi.org/10.1007/978-1-60327-185-1_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-184-4

  • Online ISBN: 978-1-60327-185-1

  • eBook Packages: Springer Protocols

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