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Generation of Full-Length cDNA Libraries: Focus on Plants

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Expressed Sequence Tags (ESTs)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 533))

Abstract

Full-length cDNAs are essential for the correct annotation of transcriptional units and gene products from genomic sequence data and for functional analysis of the genes. Full-length cDNA libraries are very important resources for isolation of the full-length cDNAs. The biotinylated cap trapper method using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.

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Acknowledgments

This work was supported by a grant for Genome Research from RIKEN, the Program for Promotion of Basic Research Activities for Innovative Biosciences, the Special Coordination Fund of the Science and Technology Agency, and a Grant-in-Aid from the Ministry of Education, Culture, Sports and Technology of Japan (MEXT) to KS. It was also supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (C) “Genome Science” from MEXT to MS and by a grant for the RIKEN Genome Exploration Research Project from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government to YH.

Author information

Authors and Affiliations

  1. Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, Yokohama, Japan

    Motoaki Seki & Asako Kamiya

  2. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Japan

    Motoaki Seki

  3. Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, Yokohama, Japan

    Piero Carninci & Yoshihide Hayashizaki

  4. Genome Science Laboratory, RIKEN, Wako, Japan

    Piero Carninci & Yoshihide Hayashizaki

  5. CREST, Japan Science and Technology Corporation (JST), Japan

    Kazuo Shinozaki

  6. RIKEN Plant Science Center, Yokohama, Japan

    Kazuo Shinozaki

Authors
  1. Motoaki Seki
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  2. Asako Kamiya
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  3. Piero Carninci
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  4. Yoshihide Hayashizaki
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  5. Kazuo Shinozaki
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Editor information

Editors and Affiliations

  1. Molecular Structure and Function Hospital for Sick Children, Departments of Biochemistry & Molecular Genetics, University of Toronto, Toronto, ON, Canada

    John Parkinson

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Seki, M., Kamiya, A., Carninci, P., Hayashizaki, Y., Shinozaki, K. (2009). Generation of Full-Length cDNA Libraries: Focus on Plants. In: Parkinson, J. (eds) Expressed Sequence Tags (ESTs). Methods in Molecular Biology, vol 533. Humana Press. https://doi.org/10.1007/978-1-60327-136-3_4

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  • DOI: https://doi.org/10.1007/978-1-60327-136-3_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-759-4

  • Online ISBN: 978-1-60327-136-3

  • eBook Packages: Springer Protocols

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