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Genetic Transformation of Carnation (Dianthus caryophylus L.)

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Abstract

This chapter describes a rapid and efficient protocol for explant preparation and genetic transformation of carnation. Node explants from greenhouse-grown plants and leaf explants from in vitro plants are infected with Agrobacterium tumefaciens AGL0 harboring pKT3 plasmid, consisting of GUS and NPTII genes. Explant preparation is an important factor to obtain the transformed plants. The GUS-staining area was located only on the cut end of explants and only explants with a cut end close to the connecting area between node and leaf, produced transformed shoots. The cocultivation medium is also an important factor for the successful genetic transformation of carnation node and leaf explants. High genetic transformation efficiency of node and leaf explants cocultured with Agrobacterium tumefaciens was achieved when the explants were cocultivated on a filter paper soaked with water or water and acetosyringone mixture (AS).

Key words:

Carnation (Dianthus caryophylus L.) Agrobacterium Genetic transformation, Adventitious shoot regeneration Node explants Leaf explant Gus (β-glucuronidase) nptII (neomycin phosphotransferase) AS (acetosyringone) 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Horticulture, Faculty of Agricultural ProductionMaejo UniversityChiangmaiThailand
  2. 2.Department of Horticulture Science, Faculty of AgricultureKagawa UniversityKagawaJapan

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