Micropropagation of Poinsettia by Organogenesis

  • Marcos Castellanos
  • J. Brian Power
  • Michael R. DaveyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 589)


Poinsettia (Euphorbia pulcherrima) is one of the most popular ornamental pot plants. Conventional propagation is by cuttings, generally focused on a period prior to the most intensive time of sales. Rapid multiplication of elite clones, the production of pathogen-free plants and more rapid introduction of novel cultivars (cvs.) with desirable traits, represent important driving forces in the poinsettia industry. In recent years, different strategies have been adopted to micropropagate poinsettia, which could assist breeders to meet consumer demands. The development of reliable in vitro regeneration procedures is likely to play a crucial role in future production systems. Stem nodal explants cultured on semi-solid MS-based medium supplemented with benzylaminopurine (BAP) and naphthalene acetic acid (NAA) develop shoots from adventitious/axillary buds after 7 weeks of culture. Rooting of in vitro regenerated shoots is achieved in semi-solid MS-based medium containing the auxin indole-3-acetic acid (IAA). Four to six weeks after transfer to root-inducing medium, regenerated plants can be transferred to compost and acclimatized in the glasshouse. Direct shoot regeneration from cultured explants is important to minimize somaclonal variation in regenerated plants.

Key words:

Euphorbia pulcherrima Ornamental plants Shoot regeneration Stem nodal explants Tissue culture 


  1. 1.
    Jerardo A (2002) Holiday sales look bright for Christmas trees and poinsettias. Agric Outlook 297:14–15Google Scholar
  2. 2.
    Clarke JL, Klemsdal SS, Floistad E, Hvoslef-Eide AK, Haugslien S, Moe R, Blystad DR (2006) Genetic engineering of poinsettia with the aim of enhancing its resistance to poinsettia mosaic virus. Acta Hortic 722:321–325Google Scholar
  3. 3.
    Jerardo A (2007) Floriculture and nursery crops yearbook. Online. Economic Research Service. United States Department of Agriculture Publications. Retrieved from Accessed 4 Feb 2008
  4. 4.
    Hartley DE (1992) Poinsettias. In: Larson RA (ed) Introduction to floriculture. Academic Press, New York, USA, pp 306–331Google Scholar
  5. 5.
    Preil W, Florak P, Wix U, Back A (1988) Towards mass propagation by use of bioreactors. Acta Hortic 226:99–107Google Scholar
  6. 6.
    Pickens KA, Cheng ZM, Trigiano RN (2005) Axillary bud proliferation and organogenesis of Euphorbia pulcherrima Winter RoseTM. In Vitro Cell Dev Bio-Plant 41:770–774CrossRefGoogle Scholar
  7. 7.
    Lin M (2006) Induction and differentiation of callus in Euphorbia pulcherrima. J Southwest Univ Sci Technol 21:109–112Google Scholar
  8. 8.
    Pickens KA, Cheng ZM, Kania SA (2006) Effects of colchicines and oryzalin on callus and adventitious shoot formation of Euphorbia pulcherrima Winter RoseTM. HortScience 41:1651–1655Google Scholar
  9. 9.
    Paludan N, Begtrup J (1986) Inactivation of poinsettia mosaic virus and poinsettia cryptic virus in Euphorbia pulcherrima using heat treated mini-cuttings and meristem-tip culture. Tidsskrift für Planteavl 90:283–290Google Scholar
  10. 10.
    Preil W (1994) In vitro culture of Poinsettia. In: Stromme E (ed) The Scientific Basis of Poinsettia Production. Advisory Service, Agricultural University of Norway, Norway, pp 49–55Google Scholar
  11. 11.
    Beck K, Rasmussen K (1996) Euphorbia pulcherrima. Methods to eliminate poinsettia mosaic virus (PnMV) and reinfection by different methods to reveal the “nature” of the branching factor. Acta Hortic 432:176–186Google Scholar
  12. 12.
    Osternack N, Saare-Surminski K, Preil W, Lieberei R (1999) Induction of somatic embryos, adventitious shoots and roots in hypocotyl tissue of Euphorbia pulcherrima Willd. ex. Klotzsch: comparative studies on embryogenic and organogenic competence. J Appl Bot 73:197–201Google Scholar
  13. 13.
    Jasrai YT, Thaker KN, D’Souza C (2003) In vitro propagation of Euphorbia pulcherrima Willd. through somatic embryogenesis. Plant Tissue Cult 13:31–36Google Scholar
  14. 14.
    Castellanos M, Power JB, Davey MR (2006) Somatic embryogenesis in red- and white-bract cultivars of poinsettia. Prop Ornamental Plants 6:61–66Google Scholar
  15. 15.
    Ying X, Gang W (2006) Studies on high quality somatic embryogenesis applied to artificial seed of Euphorbia pulcherrima Willd. Acta Hortic Sin 33:175–178Google Scholar
  16. 16.
    Murashige T, Skoog F (1962) A revised medium for the rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–496CrossRefGoogle Scholar
  17. 17.
    Geier T, Beck A, Preil W (1992) High uniformity of plants regenerated from cytogenetically variable embryogenic suspension cultures of poinsettia (Euphorbia pulcherrima Willd. ex. Klotzsh). Plant Cell Rep 11:150–154CrossRefGoogle Scholar
  18. 18.
    Wilkerson D, Barnes L, Drees B, Hall C (1999) The Texas poinsettia producers guide. Online. Texas Agricultural Extension Service, Texas A&M. Retrieved from Accessed 30 Jan 2008
  19. 19.
    Roy SK, Jinnah M (2001) In vitro micropropagation of poinsettia (Euphorbia pulcherrima Willd.). Plant Tissue Cult 11:133–140Google Scholar
  20. 20.
    Kristoffersen T (1994) Early Norwegian studies of growth and development in poinsettia. In: Stromme E (ed) The Scientific Basis of Poinsettia Production. Advisory Service, Agricultural University of Norway, Norway, pp 15–28Google Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Marcos Castellanos
    • 1
  • J. Brian Power
    • 1
  • Michael R. Davey
    • 1
    Email author
  1. 1.Plant and Crop Sciences Division, School of BiosciencesUniversity of NottinghamLoughboroughUK

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