Protocols for In Vitro Propagation of Ornamental Plants

Volume 589 of the series Methods in Molecular Biology pp 303-324


In Vitro Conservation and Cryopreservation of Ornamental Plants

  • Elif Aylin OzudogruAffiliated withDepartment of Biology, Laboratory of Plant Tissue Culture, Gebze Institute of Technology
  • , Alberto PreviatiAffiliated withCentro Sperimentale Ortofloricolo “Po di Tramontana”, Veneto Agricoltura
  • , Maurizio LambardiAffiliated withIVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, National Research Council (CNR) Email author 

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Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage) or in liquid nitrogen (cryopreservation) of plant material. Slow growth storage refers to the techniques enabling the in vitro conservation of shoot cultures in aseptic conditions by reducing markedly the frequency of periodic subculturing, without affecting the viability and regrowth of shoot cultures. Cryopreservation refers to the storage of explants from tissue culture at ultra-low temperature (−196°C). At such temperature, all the biological reactions within the cells are hampered, hence the technique makes available the storage of plant material for theoretically unlimited periods of time. An exhaustive review of papers dealing with the slow growth storage and the cryopreservation of ornamental species is reported here. Step-by-step protocols for the slow growth storage of rose germplasm, the production of synthetic seeds for the in vitro conservation of ornamentals, and the cryopreservation of Chrysanthemum morifolium are included.

Key words

Cryopreservation In vitro conservation Liquid nitrogen Slow growth storage Synthetic seeds