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Clonal Propagation of Cyclamen persicum Via Somatic Embryogenesis

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Abstract

Cyclamen (Cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. Starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(γ,γ-dimethylallylamino)purine (2iP). Transfer to hormone-free medium results in the differentiation of somatic embryos, which afterwards germinate on the same medium. These first culture stages take about 6–7 months and are carried out in complete darkness. Two to four months after the transfer to light, plantlets develop which can be acclimatized in the greenhouse. The regenerated plants are characterized by low percentages of somaclonal variation. This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.

Key words

In vitro Multiplication Ornamental Regeneration Somaclonal variation 
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Notes

Acknowledgments

The author is very grateful to Dr. Hans-Georg Schwenkel who was the first to develop somatic embryogenesis in cyclamen starting from ovules and who introduced me into the system. Furthermore, many thanks go to my students working on cyclamen, mainly Dr. Annette Hohe, Dr. Anke Pueschel, Dr. Viola Mußmann, Dr. Lara Meyer, Dr. Agnieszka Ilczuk, Antje Doil, Anika Prange, Janine Specht, and Christina Rode.

References

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Institute of Floriculture and Woody Plant Science, Tree Nursery Science Section, Leibniz University HannoverHannoverGermany

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