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In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers

  • Sabita Bhattacharya
  • Sanghamitra Bhattacharyya
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 589)

Abstract

The growing demand for flower extracts in perfume trade can primarily be met by increasing flower production and multiplying planting material. The major commercial aromatic flower yielding plants including Jasminum officinale L., a member of the Family Oleaceae have drawn the attention of a large section of the concerned sectors leading to a thrust upon developing advanced propagation technologies for these floral crops, in addition to conventional nature-dependent agro-techniques. This chapter describes concisely and critically, a protocol developed for in vitro propagation of Jasminum officinale by shoot regeneration from existing as well as newly developed adventitious axillary buds via proper phytohormonal stimulation. To start with nodal segments as explants, March–April is the most ideal time of the year when planting material suitable for in vitro multiplication is abundantly available. Prior to inoculation of explants in the culture medium, special care is needed to reduce microbial contamination by spraying on selected spots of the donor plant with anti-microbial agents 24 h prior to collection; treatment with antiseptic solution after final cleaning and surface sterilization by treating explants with mercuric chloride. Inoculated explants are free from brown leaching from cut ends by two consecutive subcultures within 48 h in MS basal medium. Multiplication of shoots, average 4–5 at each node, takes place in MS medium containing 4.0 mg/L BAP, 0.1 mg/L NAA, and 40 g/L sucrose over a period of 8 weeks. For elongation of regenerated shoots, cultures are transferred to MS medium, supplemented with a single growth hormone, kinetin at 2.0 mg/L. Emergence and elongation of roots from shoot base is facilitated by placing on the notch of a filter paper bridge. The hardened in vitro propagated plants are able to grow normally in soil like other conventionally propagated Jasminum officinale.

Key words:

Jasminum officinale Sterilization Phenolic leaching Axillary shoot Rooting of shoots Hardening 

References

  1. 1.
    Dadlani NK (1998) Cut flower production in India. In: Cut flower production in Asia. RAP Publ.Google Scholar
  2. 2.
    Kumar GS, Jayaveera KN, Ashok Kumar CK, Sanjay UP, Vrusabendra Swamy BM, Kishore Kumar DV (2007) Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop J Pharm Res 6:717–723Google Scholar
  3. 3.
    Brown D (1995) Encyclopedia of herbs and their uses. Dorling, Kindersley, London, ISBN 0-7513-020-31Google Scholar
  4. 4.
    Iqbal M, Ghosh AKM, Saluja AK (2006) Antifertility activity of floral buds of Jasminum officinale var. grandiflorum in rats. Phytotherapy Res 7:5–8CrossRefGoogle Scholar
  5. 5.
    Duke JE, Ayensu ES (1985) Jasminum officinalis. In: Medicinal plants of China. Reference Publ.Google Scholar
  6. 6.
    Bajaj YPS (ed) (1089) Biotechnology in agriculture and forestry, Vol.7 Medicinal and aromatic plants II. Springer, Berlin, Heidelberg, New YorkGoogle Scholar
  7. 7.
    George D, Chenieux JC, Ochatt SJ (1993) Plant regeneration from aged callus cultures of woody ornamental species of Lonicera japonica cv. “Hall’s Prolific”. Plant Cell Rep 13:91–94CrossRefGoogle Scholar
  8. 8.
    Ochatt SJ (1991) Requirement for plant regeneration from protoplasts of the shrubby ornamental honey suckle Lonicera nitida cv. Maigram. Plant Cell Tiss Org Cult 25:161–167Google Scholar
  9. 9.
    Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose – a review. Biotech Adv 24:94–114CrossRefGoogle Scholar
  10. 10.
    Khoder M, Villemur P, Jonard R (1979) Micropropagation et bouturage in vitro chez le Jasmine (Jasminum officinale L.). CR Acad Sci Paris Ser D 293:343–346Google Scholar
  11. 11.
    Jonard R (1989) Jasminum spp. (Jasmine): Micropropagation and the production of essential oils. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. medicinal and aromatic plants II, Vol. 7. Springer-Verlag, Berlin-Heidelberg, New York, pp 315–331Google Scholar
  12. 12.
    Bhattacharya S, Bhattacharyya S (1997) Rapid multiplication of Jasminum officinale L., in vitro culture of nodal explants. Plant Cell Tiss Org Cult 51:57–60CrossRefGoogle Scholar
  13. 13.
    Sahoo Y, Chand PK (1998) Micropropagation of Vitex negundo L. woody aromatic medicinal shrub, through high frequency axillary shoot proliferation. Plant Cell Rep 18:301–307CrossRefGoogle Scholar
  14. 14.
    Ruzic DjV, Vujovic TI (2008) The effect of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). Hort Sci (Prague) 35:12–21Google Scholar
  15. 15.
    George FE (1993) Plant propagation by tissue culture, Part-2. Exegetics Ltd, UKGoogle Scholar
  16. 16.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays witVh tobacco tissue cultures. Phyisol Plant 15:473–497CrossRefGoogle Scholar
  17. 17.
    Miller CO (1967) Cytokinins in Zea mays. Ann NY Acad Sci 144:250–257CrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sabita Bhattacharya
    • 1
  • Sanghamitra Bhattacharyya
    • 2
  1. 1.Department of BotanyBose InstituteKolkataIndia
  2. 2.Department of Molecular CardiologyCleveland ClinicClevelandUSA

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