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Structural Proteomics pp 537–559Cite as

Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 426))

 

The focus over the last several years on increasing the number of three-dimensional structures of macromolecules by implementation of high throughput methodology has led to the establishment of dedicated structural genomics programs around the world. These worldwide efforts have in turn led to development of novel, parallelized approaches to cloning, expression, purification, and crystallization of proteins. This chapter describes in some detail the approaches and protocols that have been implemented in the Bacterial Structural Genomics Initiative.

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Acknowledgments

The authors acknowledge the important contributions of a number of lab members, both past and present, to the procedures and protocols described in this chapter, including Yunge Li, Véronique Sauvé, Christine Munger, Eunice Ajamian, Erumbi S. Rangarajan, Pietro Iannuzzi, Guy Nadeau, Robert Larocque, Patrice Bouchard, Stephane Raymond, Jayaraman Sivaraman, Nicholas O'Toole, Joseph D. Schrag, Vladimir V. Lunin, Gurvan Michel, Joao Barbosa, Ante Tocilj, and Frederic Ouelette.

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Authors and Affiliations

  1. Biotechnology Research Institute, National Research Council Canada, Montreal, Canada

    Miroslaw Cygler, Ming-ni Hung, John Wagner & Allan Matte

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  1. Miroslaw Cygler
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  2. Ming-ni Hung
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  3. John Wagner
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  4. Allan Matte
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Editors and Affiliations

  1. School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland, Australia

    Bostjan Kobe

  2. School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia

    Mitchell Guss

  3. School of Molecular and Microbial Sciences and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia

    Thomas Huber

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Cygler, M., Hung, Mn., Wagner, J., Matte, A. (2008). Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination. In: Kobe, B., Guss, M., Huber, T. (eds) Structural Proteomics. Methods in Molecular Biology™, vol 426. Humana Press. https://doi.org/10.1007/978-1-60327-058-8_36

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  • DOI: https://doi.org/10.1007/978-1-60327-058-8_36

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-809-6

  • Online ISBN: 978-1-60327-058-8

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