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Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening

  • Muthuswamy Balasubramanyam
  • Antonysunil Adaikalakoteswari
  • Zaheer Sameermahmood
  • Viswanathan Mohan
Part of the Methods in Molecular Biology book series (MIMB, volume 610)

Abstract

Oxidative stress is fast becoming the nutritional and medical buzzword for the twenty-first century. The theoretical importance of oxidative stress in diabetes is highlighted by its potential double impact on metabolic dysfunction on one hand and the vascular system on the other hand. The new concept of oxidative stress, being an important trigger in the onset and progression of diabetes and its complications, emphasizes the need for measurement of markers of oxidation to assess the degree of oxidative stress. While we have been routinely measuring biomarkers in our molecular epidemiology projects, here we discuss the utility of two assays, (a) DNA damage assessment by COMET measurement and (b) telomere length measurement. As DNA damage is efficiently repaired by cellular enzymes, its measurement gives a snapshot view of the level of oxidative stress. The protocol allows for measurement of oxidative DNA damage (FPG-sensitive DNA strand breaks). Telomere length measured by Southern blotting technique allows one to estimate the chronic burden of oxidative stress at the molecular level and is now considered as biomarker of biological aging.

Key words

Oxidative stress telomere shortening comet assay DNA damage biomarker diabetes 

Notes

Acknowledgments

This work was supported by research grants from the Department of Science and Technology (DST & DST-FIST), the Department of Biotechnology (DBT), and the Indian Council of Medical Research (ICMR), Government of India.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Muthuswamy Balasubramanyam
    • 1
  • Antonysunil Adaikalakoteswari
    • 2
  • Zaheer Sameermahmood
    • 2
  • Viswanathan Mohan
    • 3
  1. 1.Department of Cell and Molecular BiologyMadras Diabetes Research Foundation & Dr Mohan’s Diabetes Specialties CentreChennaiIndia
  2. 2.Department of Cell and Molecular BiologyMadras Diabetes Research Foundation & Dr Mohan’s Diabetes SpecialtiesChennaiIndia
  3. 3.Madras Diabetes Research Foundation & Dr Mohan’s Diabetes Specialties CentreChennaiIndia

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