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Generation of Transgenic Frogs

  • Jana Loeber
  • Fong Cheng Pan
  • Tomas Pieler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 561)

Summary

The possibility of generating transgenic animals is of obvious advantage for the analysis of gene function in development and disease. One of the established vertebrate model systems in developmental biology is the amphibian Xenopus laevis. Different techniques have been successfully applied to create Xenopus transgenics; in this chapter, the so-called meganuclease method is described. This technique is not only technically simple, but also comparably efficient and applicable to both Xenopus laevis and Xenopus tropicalis. The commercially available endonuclease I-SceI (meganuclease) mediates the integration of foreign DNA into the frog genome after coinjection into fertilized eggs. Tissue-specific gene expression, as well as germline transmission, has been observed.

Key words

Xenopus laevis Transgenesis Meganuclease I-SceI Germline transmission Tissue-specific promoter 

Notes

Acknowledgments

This work was supported by funds from the Deutsche Forschungsgemeinschaft to T.P.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jana Loeber
    • 1
  • Fong Cheng Pan
    • 2
    • 3
  • Tomas Pieler
    • 1
  1. 1.Department of Developmental BiochemistryUniversity of GoettingenGoettingenGermany
  2. 2.Vanderbilt University Program in Developmental Biology3144A MRBIII Vanderbilt University Medical CenterNashvilleTN
  3. 3.Department of Cell and Biology3144A MRBIII Vanderbilt University Medical CenterNashvilleTN

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