Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains

  • Martin D. Fray
Part of the Methods in Molecular Biology book series (MIMB, volume 561)


The mouse is now firmly established as the model organism of choice for scientists studying mammalian biology and human disease. Consequently, a plethora of novel, genetically altered (GA) mouse lines have been created. In addition, the output from the large scale mutagenesis programmes currently under way around the world will increase the collection of GA mouse strains still further. Because of the implications for animal welfare and the constraints on resources, it would be unreasonable to expect anything other than those strains essential for ongoing research programmes to be maintained as breeding colonies. Unfortunately, unless the redundant strains are preserved using robust procedures, which guarantee their recovery, they will be lost to future generations of researchers.

This chapter describes some of the preservation methods currently used in laboratories around the world to archive novel mouse strains.

Key words

Cryopreservation Archive Mouse Spermatozoa Embryos Ovary Vitrification Freeze-drying 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Martin D. Fray
    • 1
  1. 1.Frozen Embryo & Sperm Archive (FESA), Medical Research CouncilMammalian Genetics UnitHarwellUK

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