Summary
We present a single-molecule method for studying protein–DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
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The help of the rest of the members of the Gene Machines’ group at Physics Department, University of Oxford, is greatly acknowledged. We would also like to thank the groups of Richard Ebright and Shimon Weiss for a fruitful collaboration on transcription-related projects. Funding was provided by the UK IRC in Bionanotechnology, the European Union (MIRG-CT-2005-031079), and the National Institutes of Health (NIH R01 GM069709). MH was supported by a fellowship from the German Academic Exchange Service (DAAD).
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Heilemann, M., Hwang, L.C., Lymperopoulos, K., Kapanidis, A.N. (2009). Single-Molecule FRET Analysis of Protein-DNA Complexes. In: Leblanc, B., Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 543. Humana Press. https://doi.org/10.1007/978-1-60327-015-1_29
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DOI: https://doi.org/10.1007/978-1-60327-015-1_29
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