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Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs

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Plant MicroRNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 592))

Abstract

Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.

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Acknowledgments

This work was supported by the Ministry of Science and Technology of China.

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Authors and Affiliations

  1. National Institute of Biological Sciences, Beijing, China

    Yijun Qi & Shijun Mi

Authors
  1. Yijun Qi
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  2. Shijun Mi
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Editor information

Editors and Affiliations

  1. Delaware Biotechnology Institute, University of Delaware, Innovation Way 15, Newark, 19711, U.S.A.

    Blake C. Meyers

  2. Delaware Biotechnology Institute, University of Delaware, Innovation Way 15, Newark, 19711, U.S.A.

    Pamela J. Green

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Qi, Y., Mi, S. (2010). Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs. In: Meyers, B., Green, P. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 592. Humana Press. https://doi.org/10.1007/978-1-60327-005-2_16

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  • DOI: https://doi.org/10.1007/978-1-60327-005-2_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-004-5

  • Online ISBN: 978-1-60327-005-2

  • eBook Packages: Springer Protocols

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