Abstract
Drosophila melanogaster imaginal discs are a widely used model system to study signal transduction, developmental, and cell biological processes. An important tool to study these processes is immunolabeling. Fluorochrome-conjugated antibody staining allows the researcher to determine the expression pattern and subcellular localization of several proteins in one imaginal disc. In this chapter, I will describe how to dissect, fix, and immunolabel imaginal discs. The protocol is streamlined so that the whole procedure can be performed in 1 d, including image acquisition.
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© 2008 Humana Press Inc., Totowa, NJ
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Klein, T. (2008). Immunolabeling of Imaginal Discs. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 420. Humana Press. https://doi.org/10.1007/978-1-59745-583-1_15
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DOI: https://doi.org/10.1007/978-1-59745-583-1_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-817-1
Online ISBN: 978-1-59745-583-1
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