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Affinity Chromatography of Phosphorylated Proteins

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 421)

Summary

This chapter covers the use of immobilized metal ion affinity chromatography (IMAC) for enrichment of phosphorylated proteins. Some requirements for successful enrichment of these types of proteins are discussed. An experimental protocol and a set of application data are included to enable the scientist to obtain high-yield results in a very short time with pre-packed phospho-specific metal ion affinity resin (PMAC).

Key Words

Phosphorylated proteins immobilized metal ion affinity chromatography ferric protein purification 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Clontech Laboratories, Inc.Mountain View

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