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Construction of High-Resolution Comparative Maps in Mammals Using BAC-End Sequences

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Phylogenomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 422))

Abstract

Whole-genome bacterial artificial chromosome (BAC) libraries for ∼40 representative species of mammals provide unique resources for the construction of high-resolution ordered genomic maps, the development of species genetics, and multispecies genome comparisons. Herein we describe procedures to construct high-quality radiation hybrid comparative maps using BAC-end sequences (BESs). This approach has been applied to the construction of ∼1 Mbp resolution porcine-human and cattle-human comparative maps. High-resolution ordered comparative maps built with BESs and whole-genome sequences have been shown to be an invaluable resource for the analysis of mammalian chromosome evolution, and as a backbone for the assembly of whole-genome shotgun sequences.

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© 2008 Humana Press Inc., Totowa, NJ

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Larkin, D.M., Lewin, H.A. (2008). Construction of High-Resolution Comparative Maps in Mammals Using BAC-End Sequences. In: Murphy, W.J. (eds) Phylogenomics. Methods in Molecular Biology™, vol 422. Humana Press. https://doi.org/10.1007/978-1-59745-581-7_6

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  • DOI: https://doi.org/10.1007/978-1-59745-581-7_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-764-8

  • Online ISBN: 978-1-59745-581-7

  • eBook Packages: Springer Protocols

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