Summary
Migration, proliferation, and collagen synthesis by vascular smooth muscle cells are thought to be key events involved in the pathogenesis of cardiovascular disease. Following endothelial injury, smooth muscle cells (SMCs) in the intima of the blood vessels assume a synthetic, pro-mitogenic phenotype resulting in their migration, proliferation, and deposition of extracellular matrix within the neointimal tissue. This chapter describes a method of isolation of SMCs from rat aorta and in vitro assays to characterize these abnormal SMC functions.
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© 2007 Humana Press Inc.
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Sreejayan, N., Yang, X. (2007). Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells. In: Sreejayan, N., Ren, J. (eds) Vascular Biology Protocols. Methods in Molecular Medicine™, vol 139. Humana Press. https://doi.org/10.1007/978-1-59745-571-8_19
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DOI: https://doi.org/10.1007/978-1-59745-571-8_19
Publisher Name: Humana Press
Print ISBN: 978-1-58829-574-3
Online ISBN: 978-1-59745-571-8
eBook Packages: Springer Protocols