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Modeling Cerebral Ischemia in Neuroproteomics

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Neuroproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 566))

Summary

Protein changes induced by traumatic or ischemic brain injury can serve as diagnostic markers as well as therapeutic targets for neuroprotection. The focus of this chapter is to provide a representative overview of preclinical brain injury and proteomics analysis protocols for evaluation and discovery of novel biomarkers. Detailed surgical procedures have been provided for inducing MCAo and implantation of chronic indwelling cannulas for drug delivery. Sample collection and tissue processing techniques for collection of blood, CSF, and brain are also described including standard biochemical methodology for the proteomic analysis of these tissues.

The dynamics of proteomic analysis is a multistep process comprising sample preparation, separation, quantification, and identification of proteins. Our approach is to separate proteins first by two-dimensional gel electrophoresis according to charge and molecular mass. Proteins are then fragmented and analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Identification of proteins can be achieved by comparing the mass-to-charge data to protein sequences in respective databases.

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Acknowledgments

Research was conducted in compliance with the Animal Welfare Act and other Federal statutes and regulations relating to animals and experiments involving animals and adheres to the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH publication 85–23. Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the authors, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense.

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Authors and Affiliations

  1. Department of Applied Neurobiology, Division of Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD, USA

    Jitendra R. Dave, Anthony J. Williams, Changping Yao, X.-C. May Lu & Frank C. Tortella

Authors
  1. Jitendra R. Dave
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  2. Anthony J. Williams
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  3. Changping Yao
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  4. X.-C. May Lu
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  5. Frank C. Tortella
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Corresponding author

Correspondence to Jitendra R. Dave .

Editor information

Editors and Affiliations

  1. Dept. Anatomy & Neurobiology, Virginia Commonwealth University School of Medicine, Richmond, U.S.A.

    Andrew K. Ottens

  2. Center of Innovative Research, Banyan Biomarkers, Inc. Center of Innovative Research, Alachua, U.S.A.

    Kevin K.W. Wang

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Dave, J.R., Williams, A.J., Yao, C., Lu, XC.M., Tortella, F.C. (2009). Modeling Cerebral Ischemia in Neuroproteomics. In: Ottens, A., Wang, K. (eds) Neuroproteomics. Methods in Molecular Biology, vol 566. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-562-6_2

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  • DOI: https://doi.org/10.1007/978-1-59745-562-6_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-84-8

  • Online ISBN: 978-1-59745-562-6

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